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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Anti HSV-1 Activity of Spirulina platensis Polysaccharide) ผู้เขียน: Nattayaporn Chirasuwan, Ratana Chaiklahan, Marasri Ruengjitchatchawalya, Boosya Bunnag,  นางมรกต ตันติเจริญ สื่อสิ่งพิมพ์:pdf AbstractAqueous extracts of Spirulina platensis were precipitated by cetyltrimethylammonium bromide (CTAB). The hot water extract was found anti Herpes simplex virus type 1 (HSV-1) activity at 50% inhibitory concentration (IC50) values of 21.32 ?g/ml. Partial purification by gel filtration of the crude extract on Sepharose 6B column gave two fractions, SHP-F1 and SHP-F2, which revealed about 4 and 2 times higher activity than that of the crude hot water extract, respectively. The crude hot water extract was a polysaccharide with rhamnose as the main sugar component. Calcium ion and sulfate groups in this polysaccharide had major roles in antiviral activity. However, the crude hot water extract polysaccharide contained approximately 42% carbohydrate and 31% protein. Decreasing the amount of protein by precipitation with trichloroacetic acid (TCA) resulted in higher purity of the crude hot water extract polysaccharide. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Response of Spirulina platensis C1 to High Temperature and High Light Intensity) ผู้เขียน:Ratana Chaiklahan, Nittaya Khonsarn, Nattayaporn Chirasuwan, Marasri Ruengjitchatchawalya, Boosya Bunnag, นางมรกต ตันติเจริญ สื่อสิ่งพิมพ์:pdf AbstractResponse of Spirulina platensis strain C1 to high temperature and high light intensity was studied. Specific growth rate of the S. platensis C1 cells grown in batch cultures at 35?C with a light intensity of 100 ?E/m2/s was 0.0247 h-1. The specific growth rate decreased to 0.008 h-1 at 43?C, which was designated to be the critical temperature for this alga strain C1. A suddenly increasing in light intensity from 100 to 500 ?E/m2/s showed an impact to the cell growth, which it had significant affects to photosynthesis (as O2-evolution) as well as its pigment content (chlorophyll and phycocyanin). However, a less effect on the alga growth was obtained when the cells were shifted to grow at higher temperature of 43?C. The alga cells grown at high temperature with high light intensity resulted in decreasing protein content while carbohydrate content increased. The fatty acid profiles of the cells grown at high temperature exhibited a decrease in polyunsaturated fatty acid (C18:3?6,9,12) while C18:2?9,12 increased. In contrast, the alga cells grown in a high light intensity showed an increase in
C18:2?9,12, while C18:1?9 and total fatty acid content (TFA) were decreased. On the other hand, the combined effects of temperature and light intensity (43?C, 500 ?E/m2/s) showed that the period of recovery in photosynthesis depends upon the period of exposure to the stress parameter. |
ผลงานตีพิมพ์ในวารสารวิชาการConstruction of Light-Responsive Gene Regulatory Network for Growth, Development and Secondary Metabolite Production in Cordyceps militarisผู้แต่ง:In-On, A., Thananusak, R., Ruengjitchatchawalya, M., Ms.Wanwipa Vongsangnak, Associate Professor , Laomettachit, T., วารสาร: 
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimum Storage Condition for Spirulina Mat before Applying to
Ammonia-Nitrogen Removal from Simulated Shrimp Culturing Water) ผู้เขียน:Krittayot Chaowanapreecha, Chalermraj Wantawin, Marasri Ruengjitchatchawalya สื่อสิ่งพิมพ์:pdf AbstractThe polyester mats fully attached by Spirulina were stored under different conditions before
using for ammonia removal from simulated shrimp culturing water. Three factors on storage were studied;
light intensity of 1000, 2000 and 3000 lux; substrate concentration of 1X, 0.5X, 0.3X and 0.1X Zarrouk’s
medium; storage time of 2, 4, and 6 weeks. During storage, detachment of excess cells due to growth
resulted in accumulation of suspended solids at 6 weeks in the range of 155 to 202 mg/l. More suitable
condition for growth during storage yielded more suspended solids in solution. Circulated batch reactor
with flow velocity of 0.14 m/s and initial ammonia nitrogen of 1 mg/l was applied for investigating the
ammonia removal by stored Spirulina mats. Steady state effluent ammonia concentration of 0.127 mg-
N/1 from fresh Spirulina mat reactor (control experiment) was achieved within 20 days. The control
relative ammonia removal efficiencies from Spirulina mats, kept six weeks before applying in 1x
Zarrouk’s medium under 3000, 2000 and 1000 lux reactor at the end of experiment (25 days), were
93.8%, 92.3% and 91.9%, respectively. While the efficiencies by Spirulina mats, under 1000 lux light
intensity, after storing in 0.5x, 0.3x and 0.lx Zarrouk’s medium were 92.0%, 91.8% and 87.9%,
respectively. Concentration of daily washed out suspended solid was found stable in all reactors. Optimal
condition for storage of Spirulina for long term period (six weeks) was 1000 lux light intensity and 0.1x
Zarrouk’s medium. |
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 งานวิจัยนวัตกรรมวิจัยเพื่อค้นหายาต้านเอดส์ (ระยะที่ 2) (2010)หัวหน้าโครงการ:ดร.สุภา หารหนองบัว, ศาสตราจารย์ ผู้ร่วมโครงการ:ดร.สุพรรณา เตชะสกุล, รองศาสตราจารย์ , ดร.วราภรณ์ พาราสุข, รองศาสตราจารย์ , ดร.พจมาน พูลมี, ผู้ช่วยศาสตราจารย์ , ดร.เกียรติทวี ชูวงศ์โกมล, รองศาสตราจารย์ , ดร.นลวัฒน์ บุญญาลัย, ผู้ช่วยศาสตราจารย์, ดร.พัชรีนารถ ทรัพย์อาภากร, รองศาสตราจารย์, ดร.วีรมลล์ ไวลิขิต, ผู้ช่วยศาสตราจารย์, ดร.ทรงวุฒิ สุรมิตร, รองศาสตราจารย์ , ดร.วิชชา ตรีสุวรรณ, ผศ.ดร.พรพรรณ พึ่งโพธิ์ แหล่งทุน:สำนักงานกองทุนสนับสนุนการวิจัย ผลลัพธ์:วารสาร (14) ประชุมวิชาการ (52) วิจัย/สิ่งประดิษฐ์ (1) |